Proteobiotics are both natural and backed by scientific research.
Summary of publications in refereed journals that pertain to proteobiotics
An in vitro study investigating the impact of the cell-free supernatant on the expression of virulence genes in enterohemorrhagic Escherichia coli O157:H7. For the full research paper, click here.
Clostridium difficile is an animal pathogen that causes diarrhea and colitis. The cell-free supernatant of Lactobacillus acidophilus has anti-infective properties and reduces the pathogenicity in a murine model challenged with Cl. difficile. For the full research paper, click here.
Campylobacter jejuni is a foodborne pathogen that causes acute diarrhea in humans. Pathogenic bacteria including C. jejuni cause disease via virulence factors. A reduction in the genetic expression of virulence factors can reduce the severity of the disease symptoms. For the full research paper, click here.
A bioluminescence bioassay was developed to quantify auto-inducer-2 (AI-2) quorum sensing signals. The cell-free supernatant of Bifidobacterium longum (ATCC 15707) reduced the activity of AI-2 98-fold. Moreover, the supernatant reduced E. coli biofilm formation by 36% without inhibiting growth. For the full research paper, click here.
Yogurt supplemented with a bioactive fraction from a Lactobacillus acidophilus (La5) fermentation has prophylactic effects with mice challenged with enterohemorrhagic E. coli. Mice were fed yogurt for 4 days prior to a challenge of E. coli O157:H7. Clinical disease symptoms were reduced in treated mice compared to the untreated control. For the full research paper, click here.
Certain Bifidobacteria species are able to reduce gene activity of regulatory and virulence systems in Salmonella enterica serovar Typhimurium and Escherichia coli O157:H7 necessary for human cell adhesion and invasion in infections with these particular pathogens. For the full research paper, click here.
The probiotic species, Lactobacillus helveticus was used to ferment milk from which a peptide fraction was isolated using a spectroscopic analysis of the concentration of the protein in the cell- free medium solution. A murine model was used to assess the peptide’s ability to increase the immune response of the mice. For the full research paper, click here.
In this study, two reporter Salmonella strains were constructed in which the regulator of SPI1, hilA (virulence gene), and the response regulator of SPI2 (ssrB) were fused with luxCDABE gene cassette, and which were used to screen the effects of the cell-free medium solution isolated from five probiotic lactic acid bacteria fermentations on the virulence of the particular Salmonella strain. For the full research paper, click here.
In this study, the ability of Lactobacillus acidophilus (strain La-5) to produce bioactive molecules to prevent the adherence and invasion of enterohemorrhagic Escherichia coli to gut epithelial cells and to also concentrate F-actin at sites of pathogenic adhesion was studied. For the full research paper, click here.
In this study the bioactive component from the Lactobacillus acidophilus (La5) fermentation was investigated. The fermentation broth was fractionated using size exclusion chromatography and the fractions were assayed using an AI-2 bioluminescence assay. For the full research paper, click here.
This study indicates that the bioactive molecules produced by L. acidophilus reduces incidence of enteric colibacillosis in pigs and their use on farms would help to reduce antibiotic use. For the full research paper, click here.